摘要
应用４种方法判定烧伤创面异体移植的培养表皮细胞存活情况：１．聚合酶链反应（ＰＣＲ）查Ｙ染色体特异性ＤＮＡ片段；２．ＰＣＲ扩增ＰＭＣＴ１１８长度多态性基因位点；３．ＰＣＲ／ＳＳＯＨＬＡ－ＤＱａ分型；４．ＤＮＡ指纹。共检测１６例病人的２７个活检标本，均证实有供者的ＤＮＡ，最长时间为移植后９２天。初步实验结果表明：培养表皮异体移植后可存活较长时间。
Four objective techniques were used for existence identification of cultured human keratinocytes allografted to different kinds of burn wounds.They include:(1) Amplification of Y chromosome specific DNA fragment by polymerase chain reaction (PCR);(2)Amplification PMCT118 polymorphous allele locus by PCR;(3) HLA DQα typing by PCR and reversed oligonucliotide hybridization;(4) DNA fingerprinting.Twenty seven allografts biopsies from 16 patients were subjected to these assays and the donor genetic marks were detected in all of these samples,among which the longest biopsy was taken at 92 days post grafting.The preliminary results of the study indicated that the cultured human epidermal allografts can survive a long period time.(Original article on page 137)
出处
《中华创伤杂志》
CAS
CSCD
北大核心
1995年第3期137-139,共3页
Chinese Journal of Trauma
基金
国家自然科学基金
关键词
聚合酶链反应
烧伤
异体移植
表皮细胞
Epidermis Cell,cultured Polymerase chain reaction Transplantation,heterologous
