摘要
作者研究了炭吸收法分离技术测定血清生长激素结合蛋白（ＧＨＢＰ）的相关因素，提出了理想的本实验室的检测条件。本法灵敏度为０．９％／１０μｌ血清，批内ＣＶ为２．５％，批间ＣＶ为６．０％。并用此法对正常青春发育期儿童１０６例，ＧＨＤ２０例，ＧＨＮＤ２４例，肥胖症１０例，Ｌａｒｏｎ型侏儒症１例，进行了血清ＧＨＢＰ测定。其中４例矮小儿童同时测夜睡眠５ｈ血清ＧＨ分泌相及ＧＨＢＰ水平的动态观察。
In this
report the authors studied the relevant factors in the dextran-coated charcoal technique for
separating the bound-hGHfrom the free-hGH,and established an optimized condition, The
sensitivity was 0.9%/10 μ1 serum. Intra-and inter-assay coeffi-cients of variation were 2.5%and
6.0%respectively. UsinR this method the authors measured the serum GHBP in 106 normal
pu-bertal children , among them 20 with GHD, 24 with GHND,10 with obesity and one with
Laron’s syndrome. In 4 children withshort-statue 5-hour night time GH and GHBP profiles were
measured.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
1994年第3期170-173,共4页
Chinese Journal of Endocrinology and Metabolism
关键词
生长激素
结合蛋白
血清诊断
Growth hormone Growth hormone-binding protein
