摘要
目的探讨通心络对大鼠脑缺血再灌注损伤的保护作用。方法将31只雄性Wistar大鼠,根据不同给药方案随机分为24h对照组(7只)、24h通心络组(7只)、5d对照组(7只)、5d通心络组(7只)以及假手术组(3只)。各组在造模前用等渗盐水或通心络灌胃预处理7d。依据线栓法建立大鼠大脑中动脉阻塞90min脑缺血再灌注模型。观察不同时间点的神经功能缺失评分,计算2,3,5氯化三苯基四氮唑染色下的梗死体积。结果脑缺血再灌注24h后的梗死体积对照组为(94.4±19.9)mm3,通心络组为(72.1±13.4)mm3;神经功能缺失评分对照组为(3.3±0.6)分,通心络组为(2.6±0.6)分。再灌注5d后的梗死体积对照组为(123.9±18.6)mm3,通心络组为(100.2±12.8)mm3;神经功能缺失评分,对照组为(2.1±0.4)分,通心络组为(1.2±0.4)分,两组比较,差异均有显著意义(P<0.01～0.05)。结论通心络对大鼠大脑中动脉阻塞后的脑缺血再灌注损伤可能具有保护作用。
Objective To investigate the effect of TongXinLuo(TXL) by dynamic changes of infarct volumes and neurological deficit scores on local cerebral ischemic-reperfusion injury in rats. Method Male Wistar rats were randomly divided into sham-operated control group(n=3),normal saline control groups (24 hours group and 5 days group) and TXL-treated groups(24 hour group and 5 day group)(n=7 each). Animals in the latter four groups were subjected to transient focal ischemia by the middle cerebral artery occlusion(MCAO) for 90 minutes. The rats were pretreated with normal saline or TXL for 7 d. At different end points, infarct volumes were determined by 2,3,5-triphenyltetrazolium chloride (TTC) and neurological scores were investigated. ~Results Infarct volumes in TXL-treated group were significantly decreased compared with those in control groups at 24 hours and 5 day after MCAO~〔(72.1±13.4)mm3 vs. (94.4±19.9)mm3,P=0.01; (100.2±12.8)mm3 vs. (123.9±~18.6)mm3 ,P<0.05,respctively〕,and the neurological deficit scores in TX-treated groups were ~synchronously improved (2.6±0.5 vs. 3.3±0.6, P<0.05;1.2±0.4 vs. 2.1±0.4,P<0.01,~respectively ).~Conclusion These results show that pretreatment with TXL can attenuate brain injury and TXL may be a potential neuroprotective agent in cerebral ischemia-reperfusion.
出处
《中国脑血管病杂志》
CAS
2005年第5期219-221,共3页
Chinese Journal of Cerebrovascular Diseases
