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AC133-2分子的克隆及其逆转录病毒表达载体的构建 被引量：1

The Cloning of the AC133-2 and Construction of Its Retroviral Expressing Vector
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摘要 目的克隆人AC133-2全长基因,构建PGEZ-Term-AC133—2逆转录病毒表达载体。方法采用分段克隆的方法,通过聚合链式反应从胎肝文库中克隆AC133-2,并构建AC133—2基因的逆转录病毒表达载体。结果成功克隆AC133—2全长基因并构建PGEZ-Term-AC133-2逆转录病毒表达载体。结论AC133-2全长基因克隆及构建逆转录病毒表达载体的成功,为构建AC133-2转基因细胞和制备抗人AC133-2单克隆抗体和研究AC133-2的生物学功能奠定了物质基础。 Objective To clone AC133-2, construct retroviral vector. Methods AC 133-2 was cloned from fetal liver. The gene was ligated with T-Vectoe and sequenced to construct retroviral vector constisting of AC133-2. Results AC133-2 and construction of retroviral vector was cloned successfully. Conclusion Successful cloning of AC133-2 and construction of retroviral vector lays the foundation for constructed the AC 133-2 transgenic cells, and preparation and characterization of anti-AC133-2 McAbs.
作者 王明元 居颂光 居颂文 狄文英 周晓华 薛群 曲静 方振羊 张学光
机构地区 苏州大学医学生物技术研究所江苏省干细胞研究重点实验室 苏州市红十字中心血站
出处 《苏州大学学报（医学版）》 CAS 北大核心 2005年第1期31-33,共3页 Suzhou University Journal of Medical Science
关键词 AC133—2基因 逆转录病毒表达载体 AC133-2 gene, retroviral vector
分类号 R392.4 [医药卫生—免疫学]
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参考文献7

  • 1De Wynter, Buck D, Hart C, et al. CD34^+ AC133^+cells isolated from cord blood are highly enriched in longterm culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors[J]. Stem Cell, 1998,16(6) :387 - 396.
  • 2Miraglia S, Godfrey W, Yin AH, et al. A novel fivetransmembrane hematopoietic stem cell antigen: isolation, characterization, and molecular cloning [ J ]. Blood,1997, 90(12): 5013 - 5021.
  • 3Yu Ying, Flint A, Dvorin EL, et al. AC133-2, a novel isoform of human AC133 stem cell antigen[J]. J Bio Chem, 2002, 277(23) :20711 - 20716.
  • 4Yin AH, Miraglia S, Esmail D, et al. AC133, a novel marker for human hematopoietic stem and progenitor cells[J]. Blood, 1997, 90(12) :5002 - 5012.
  • 5Wuchter C, Ratei R, Spahn G, et al. Impact of CD133(AC133) and CD90 expression analysis for acute leukemia immunophenotyping. Haematologica, 2001, 86(2):154- 161.
  • 6Baersch G, Baumann M, Ritter J, et al. Expression of AC133 and CD117 on candidate normal stem cell populations in childhood B-cell precursor acute lymphoblastic leukaemia[J]. Br J Haematol, 1999, 107(3):572- 580.
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同被引文献7

  • 1王伟,汪洪毅,赵会霞,崔中光,李广伦.CD133抗原在白血病及MDS骨髓细胞中的表达[J].中国实验血液学杂志,2007,15(3):470-473. 被引量：4
  • 2Wu Y,Wu PY. CD133 as a marker for cancer stem cells: progresses and concerns[J]. Stem Cells Dev,2009,18(8) : 1127-1134.
  • 3Miraglia S,C, odfrey W,Yin AH,et al. A novel five-transmembrane hematopoietic stem cell antigen: isolation,characterization,and molecular cloning[JJ. Blood, 1997,90( 12 ) :5013-5021.
  • 4Shmelkov SV,St Clair R,Lyden D,et al. AC133/CD133/Prominin-1 [J] . Int J Biochem Cell Bio,2005,37(4):715-719.
  • 5Fargeas CA,Corbeil D,Huttner WB. AC133 antigen,CD133,prominin- 1,prominin-2,etc.: prominin family gene products in need of a rational nomenclature[ J ]. Stem Cells, 2003,21(4) : 506-508.
  • 6Yu Y,Flint A,Dvofin EL,et al. AC133-2,a novel isoform of human AC133 stem cell antigen[J] . J Biol Chem,2002,277(23):20711- 20716.
  • 7de Wynter EA,Buek D,Hart C,et al. CD34+AC133+ cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors[J]. Stem Cells, 1998,16(6) : 387-396.

引证文献1

苏州大学学报（医学版）
2005年 第1期

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