摘要
用黑菇多糖体外处理人单核细胞性白血病细胞分化模型Ｕ９３７细胞，结果表明黑菇多糖明显抑制Ｕ９３７细胞体外增殖（ＩＣ５０２８０μｇ／ｍｌ）并诱导细胞向终末方向分化。经黑菇多糖诱导后的Ｕ９３７细胞，细胞形态发生显著变化，细胞膜标志和生化特征趋向成熟。
This investigation demonstrated that Pleurotus sapidus Polysaccharide(PPS)could inhibit the proliferation (IO50=280ug/ml) and induce terminal differentiation of U937 cell in vitro. After 3 days of incubation in medium containing 250μg/ml PPS,the U937 cells showed remarkable morphological changes,over 53% of the cells showed the ability to reduce non-specific esterase activity(ANAE positive),73.5% and59.5% of the cells expressed FcR and C3R respectively. PPS could also increase the activity of low density lipoprotein receptors on U937 cell surface. However, too high concentration of PPS could decreased ANAE and 125I-LDL binding activity.
出处
《上海免疫学杂志》
CSCD
北大核心
1994年第2期71-75,共5页
Shanghai Journal of Immunology
关键词
黑菇多糖
U937细胞
诱导分化
Pleurotus sapidus polysaccharide
U937 cell
Induction of differentiation
