摘要
本实验利用大鼠甩尾测痛结合脊髓蛛网膜下腔注药的方法,观察了脊髓水平内源性阿片样物质(OLS)对5一羟色胺(5—HT)4痛作用的影响。实验结果显示;(1)脊髓蛛网膜下腔注射60nmol的5—HT可产生明显的镇痛作用,并可为同一途径注射阿片受体阻断剂纳络酮所完全阻断;(2)同一剂量的纳络酮也能阻断鞘内注射60nmol吗啡所引起的镇痛作用;(3)单独使用120nmol纳络酮对痛阈无明显影响。以上结果表明.在脊髓水平5—HL的镇痛作用是通过OLS介导的。
The effect of naloxenA on the analgesia produced by intrathecally administered(i.t)serotonin(5-HT)or morphine was observed and compared in rats using tail-flick test.The results showed that:(1)The analgesia produced by 5-HT(60nmol,i,t.)could be blocked by nalox-one(120nmol,i.t.);(2)The analgesia produced by morphine(60nmol,i.t)could also be blocked by the same dosage of naloxone;(3)120nmol naloxone administration alone had no effect on the tail-flick latency;This suggests that 5-HT-induced analgesia is mediated by endogenous opiate-like substances(OLS)at the spinal level.
作者
王瑞
杨俊曾
Wang Rui(Department of Physiology,Shanxi Medical College)
出处
《长治医学院学报》
1994年第4期291-295,共5页
Journal of Changzhi Medical College
关键词
吗啡
纳络酮
血清素
阿片样物质
tail-flick latency
serotonin
morphine
naloxone
intrathecal injection
