摘要
应用单克隆抗体和免疫组化ABC法对53例肺癌的局部免疫状态及β_2微球蛋白(简称β_2 M)表达进行研究。结果表明:①机体对肺癌组织以细胞免疫为主,局部反应主要为T细胞,伴少量巨噬细胞和B细胞。免疫细胞主要分布于肺癌间质,仅少数散在于癌巢中。②肺癌局部T细胞多处于非活化状态。OKT_4/OKT_8比值各例不同,平均为1.24±0.59。③有淋巴结转移的肺癌组,其局部T细胞和活化T细胞数低于无转移组,而巨噬细胞则是转移组高于无转移组。鳞癌中活化T细胞和巨噬细胞高于未分化癌。以上差别均有显著或极显著意义。④肺癌分化好者β_2 M表达强,分化差者表达弱。β_2 M的表达与全T,辅助T和活化T细胞的浸润呈正相关。本文认为肺癌组织中免疫细胞的浸润及其活性状态可作估计预后的指标之一。β_2 M表达可做肺癌分级的参考指标。
Fifty-three cases of lung carcinoma were studied with monoclonal antibodies by using ABC immunohistochemical method Our experiment showed:(J)The infiltrated mononuclear celb in the lung carcinoma were mainly Tlymphocytes with small amount of B cells and macrophages, which distributed mainly in the stroma of lung carcinoma. Most of T cells were non-activated. The average ratio of OKT4/OKT8 was 1.24±0.59.(2) The pan T and the activated T cells in the group of lung cancer without metastasis to lymph node were more tthan that with metastask Contrary, there were more macrophages in metastatb group than that in non-metastatic group. The activated T cells and macrophages in squamous carcinoma were more than that in undifferentia ted carcinoma. All the differences showed statistical significance.(3 ) The intensity of expression of beta-2 microglobulin in lung carcinoma showed a positive correlation with differentiation of lung carcinoma and infiltration of pan T, helper T and activated T cells So the mononuclear infiltration and their activation in king cancer are significant for clinic and prognosis. Beta-2 microglobulin may be used as a referent indiacator of the grade of lung carcinoma.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1993年第4期291-294,T000,共5页
Chinese Journal of Cancer
关键词
β2
微球蛋白
肺肿瘤
免疫学
Mononuclear cell Beta-2 micro globulin Immunohis to chemistry
