摘要
〔目的〕通过实验查明造成此次疫情流行的病原菌 ,分析影响检验结果的各种原因。〔方法〕采用血、骨髓、粪便病原菌培养方法、生化反应、血清凝集实验、肥达氏反应、抗原酶联免疫实验方法和药物敏感性试验。〔结果〕由于患者血、骨髓、粪便中培养出伤寒杆菌 ,且经电子显微镜检测和L型细菌培养证实该流行菌株发生了L型变异 ,证明此疫情为L型伤寒流行 ,但疫区水源中未检出病原菌 ,肥达氏反应和酶联免疫抗原检测阳性率较低。〔结论〕本次流行菌株对青霉素、头孢哌酮等抗菌素耐药。须高度重视L型伤寒菌株的实验室检测。
〔Objective〕 To study the pathogenic bacteria during the epidemic through the test and analyze the causes for interference of the testing results.〔Methods〕 The pathogenic bacteria were cultured using the blood and bone marrow excrement biochemistry,serum agglutinin test,Widal reaction,ELISA and medicine sensitivity test.〔Results〕 Type L typhoid bacillus was discovered in the patient's blood and bone marrow.It was not found in water samples from the epidemic region.However,the detectable rate of the bacterium was low by Widal reaction and ELISA.〔Conclusions〕 The epidemic strain of type L typhoid bacillus is resistant to some antibiotics such as penicillin and cefoperazone.The laboratory test of type L typhoid bacillus should be paid close attention to.
出处
《中国卫生检验杂志》
CAS
2004年第4期449-450,共2页
Chinese Journal of Health Laboratory Technology
